Polyamine replacement by magnesium ions in BHK-21/C13 cells
نویسندگان
چکیده
منابع مشابه
Induction of partial mitosis in BHK cells by 2-aminopurine.
The protein kinase inhibitor 2-aminopurine (2-AP) inhibits a subset of mitotic events in BHK cells. In the presence of the drug, these cells form a bipolar spindle in mitosis, but chromatin fails to generate functioning chromosomes. Cells in 2-AP progress through a partial mitosis, in which there is no observable metaphase, anaphase or telophase events. After 12 h of exposure to 2-AP the chroma...
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Several aspects of the behaviour of polyoma virus-transformed BHK cells in culture have suggested that they are more deformable than BHK cells. This possibility was tested by applying negative pressure at the cell surface by means of a micropipette. It was found that PyBHK cells in early mitosis are twice as deformable as BHK cells in the same stage. In addition, the taut, non-ruffling margins ...
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Introduction The cell concentration of a polyamine depends on both its rates of synthesis and degradation and on its rates of uptake and release. A system for the uptake of putrescine, spermidine and spermine is possessed by many types of cell [ 1 1 , and the uptake of a particular polyamine is often stimulated when its synthesis is inhibited [2]. The transport system is thus involved in the re...
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The binding of plasmid DNA to norfloxacin, a quinolone antibacterial agent, was investigated by fluorescence, electrophoretic DNA unwinding, and affinity chromatography techniques. The amount of quinolone bound to DNA was modulated by the concentration of Mg2+. No interaction was evident in the absence of Mg2+ or in the presence of an excess of Mg2+, whereas maximum binding was observed at a Mg...
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A large proportion (60-85%) of the actin present in lysates of cultured baby hamster kidney (BHK) cells is monomeric at low temperature. All this G-actin is accounted for in the fractions obtained by high-speed centrifugation of the lysate in the presence or absence of non-ionic detergent; 98 (+/- 7)% of its reacts as monomer in the deoxyribonuclease assay. The fractions nevertheless possess ac...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1979
ISSN: 0306-3283
DOI: 10.1042/bj1780391